解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Previous analyses of labelled clones of cells within the developing nervous system of the mouse have indicated that descendants are initially dispersed rostrocaudally followed by more local proliferation, which is consistent with the progressing node's contributing descendants from a resident population of progenitor ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35078535
更新日期:2001-06-01 00:00:00
abstract::Etk/BMX, a member of the Btk family of tyrosine kinases, is highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines. Here, we present evidence that Etk is involved in integrin signalling and promotes cell migration. The activation of Etk by extracellula...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35074500
更新日期:2001-05-01 00:00:00
abstract::We have designed new non-peptidic potential inhibitors of gamma-secretase and examined their ability to prevent production of amyloid-beta 40 (Abeta40) and Abeta42 by human cells expressing wild-type and Swedish-mutant beta-amyloid precursor protein (betaAPP). Here we identify three such agents that markedly reduce re...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35074581
更新日期:2001-05-01 00:00:00
abstract::Different sites of plasma membrane attachment may underlie functional differences between isoforms of Ras. Here we show that palmitoylation and farnesylation targets H-ras to lipid rafts and caveolae, but that the interaction of H-ras with these membrane subdomains is dynamic. GTP-loading redistributes H-ras from raft...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35070050
更新日期:2001-04-01 00:00:00
abstract::Two forms of genetic instability have been described in colorectal cancer: microsatellite instability and chromosomal instability. Microsatellite instability results from mutations in mismatch repair genes; chromosomal instability is the hallmark of many colorectal cancers, although it is not completely understood at ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35070129
更新日期:2001-04-01 00:00:00
abstract::The guanosine tri-phosphatase Ran stimulates assembly of microtubule spindles. However, it is not known what aspects of the microtubule cytoskeleton are subject to regulation by Ran in mitosis. Here we show that Ran-GTP stimulates microtubule assembly by increasing the rescue frequency of microtubules three- to eightf...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35060000
更新日期:2001-03-01 00:00:00
abstract::Correct placement and orientation of the mitotic spindle is essential for segregation of localized components and positioning of daughter cells. Although these processes are important in many cells, few factors that regulate spindle placement are known. Previous work has shown that GPB-1, the Gbeta subunit of a hetero...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35060092
更新日期:2001-03-01 00:00:00
abstract::To study the role of the BH3 domain in mediating pro-apoptotic and anti-apoptotic activities of Bcl-2 family members, we identified a series of novel small molecules (BH3Is) that inhibit the binding of the Bak BH3 peptide to Bcl-xL. NMR analyses revealed that BH3Is target the BH3-binding pocket of Bcl-xL. Inhibitors s...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35055085
更新日期:2001-02-01 00:00:00
abstract::Coordination of microtubules and the actin cytoskeleton is important in several types of cell movement. mDia1 is a member of the formin-homology family of proteins and an effector of the small GTPase Rho. It contains the Rho-binding domain in its amino terminus and two distinct regions of formin homology, FH1 in the m...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35050598
更新日期:2001-01-01 00:00:00
abstract::Here we show that segregation of homologous chromosomes and that of sister chromatids are differentially regulated in Xenopus and possibly in other higher eukaryotes. Upon hormonal stimulation, Xenopus oocytes microinjected with antibodies against the anaphase-promoting complex (APC) activator Fizzy or the APC core su...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35050607
更新日期:2001-01-01 00:00:00
abstract::Chromatin structure is thought to play a critical role in gene expression. Using the lac operator/repressor system and two colour variants of green fluorescent protein (GFP), we developed a system to visualize a gene and its protein product directly in living cells, allowing us to examine the spatial organization and ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35046510
更新日期:2000-12-01 00:00:00
abstract::Here we show that human Zeste White 10 (Zw10) and Rough deal (Rod) are new components of the mitotic checkpoint, as cells lacking these proteins at kinetochores fail to arrest in mitosis when exposed to microtubule inhibitors. Checkpoint failure and premature mitotic exit may explain why cells defective for hZw10 and ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35046598
更新日期:2000-12-01 00:00:00
abstract::Whereas total loss of Lis1 is lethal, disruption of one allele of the Lis1 gene results in brain abnormalities, indicating that developing neurons are particularly sensitive to a reduction in Lis1 dosage. Here we show that Lis1 is enriched in neurons relative to levels in other cell types, and that Lis1 interacts with...
journal_title:Nature cell biology
pub_type: 评论,杂志文章
doi:10.1038/35041000
更新日期:2000-11-01 00:00:00
abstract::Here we evaluate the idea that the Golgi is in dynamic equilibrium with the endoplasmic reticulum (ER). In cytoplasts that lack the Golgi apparatus, no regrowth of the Golgi is observed, nor is any transport from the ER to the cell surface detected. However, introduction of the smallest measurable amount of Golgi (equ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35041089
更新日期:2000-11-01 00:00:00
abstract::Only a few RFamide peptides have been identified in mammals, although they have been abundantly found in invertebrates. Here we report the identification of a human gene that encodes at least three RFamide-related peptides, hRFRP-1-3. Cells transfected with a seven-transmembrane-domain receptor, OT7T022, specifically ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35036326
更新日期:2000-10-01 00:00:00
abstract::In response to DNA damage, eukaryotic cells use a system of checkpoint controls to delay cell-cycle progression. Checkpoint delays provide time for repair of damaged DNA before its replication in S phase and before segregation of chromatids in M phase. The Cds1 (Chk2) tumour-suppressor protein has been implicated in c...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35036406
更新日期:2000-10-01 00:00:00
abstract::Rad23 is a nucleotide-excision repair protein with a previously unknown biochemical function. We determined that yeast and human Rad23 inhibited multi-ubiquitin (Ub) chain formation and the degradation of proteolytic substrates. Significantly, Rad23 could be co-precipitated with a substrate that contained a short mult...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35023547
更新日期:2000-09-01 00:00:00
abstract::Recent theoretical analysis of a model lattice of interacting transmembrane receptor proteins has indicated that such clustering in the membrane could provide a novel mechanism for regulating receptor signalling in cells. It has been calculated that cooperative interactions between receptors organized into a cluster, ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35023625
更新日期:2000-09-01 00:00:00
abstract::The Drosophila fruitless (fru) gene product Fru has been postulated to be a neural sex-determination factor that directs the development of at least two male-specific characteristics, namely courtship behaviour and formation of the muscle of Lawrence (MOL). The fru gene encodes a putative transcription factor with a B...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35019537
更新日期:2000-08-01 00:00:00
abstract::Historically, the late arrival of optical microscopy is something of a puzzle, although it may be a testament to the fact that the development of new technology is driven by the desire for scientific knowledge, and not the other way around. The explorations that began in the 17th century are by no means complete, but ...
journal_title:Nature cell biology
pub_type: 历史文章,杂志文章
doi:10.1038/35019639
更新日期:2000-08-01 00:00:00
abstract::Wiskott-Aldrich syndrome protein (WASP) and N-WASP have emerged as key proteins connecting signalling cascades to actin polymerization. Here we show that the amino-terminal WH1 domain, and not the polyproline-rich region, of N-WASP is responsible for its recruitment to sites of actin polymerization during Cdc42-indepe...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35017080
更新日期:2000-07-01 00:00:00
abstract::Inactivation of the von Hippel-Lindau (VHL) tumour-suppressor protein (pVHL) is associated with the von Hippel-Lindau cancer syndrome and the majority of kidney cancers. New evidence suggests that pVHL has properties of an F-box protein that targets the alpha-subunits of hypoxia-inducible factor (HIF)-1 for oxygen-dep...
journal_title:Nature cell biology
pub_type: 评论,新闻
doi:10.1038/35017129
更新日期:2000-07-01 00:00:00
abstract::Cell-cell adhesion mediated by specific cell-surface molecules is essential for multicellular development. Here we quantify de-adhesion forces at the resolution of individual cell-adhesion molecules, by controlling the interactions between single cells and combining single-molecule force spectroscopy with genetic mani...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35014000
更新日期:2000-06-01 00:00:00
abstract::Compartmentalization has long been known to have a key role in regulation of cellular processes. By keeping enzymes and regulatory complexes in compartments where the delivery of substrate or exit of product is controlled, competing reactions can occur simultaneously in different parts of the cell. Moreover, spatial c...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/35014078
更新日期:2000-06-01 00:00:00
abstract::When co-translationally inserted into endoplasmic reticulum (ER) membranes, newly synthesized proteins encounter the lumenal environment of the ER, which contains chaperone proteins that facilitate the folding reactions necessary for protein oligomerization, maturation and export from the ER. Here we show, using a tem...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35010558
更新日期:2000-05-01 00:00:00
abstract::The vertebrate neural crest arises at the border of the neural plate during early stages of nervous system development; however, little is known about the molecular mechanisms underlying neural crest formation. Here we identify a secreted protein, Noelin-1, which has the ability to prolong neural crest production. Noe...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35008643
更新日期:2000-04-01 00:00:00
abstract::Examination of the tumour suppressor adenomatous polyposis coli (APC) has shown that it may be multifunctional. Recent work has demonstrated dynamic interactions of APC with the microtubule cytoskeleton, supporting the idea that APC has an important function in cell migration. ...
journal_title:Nature cell biology
pub_type: 新闻,评审
doi:10.1038/35008685
更新日期:2000-04-01 00:00:00
abstract::Mouse embryos with a loss-of-function mutation in the gene encoding the receptor tyrosine kinase ErbB4 exhibit misprojections of cranial sensory ganglion afferent axons. Here we analyse ErbB4-deficient mice, and find that morphological differences between wild-type and mutant cranial ganglia correlate with aberrant mi...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/35000058
更新日期:2000-02-01 00:00:00
abstract::When cells enter mitosis with DNA that is unfit to be segregated, the consequences appear to be loss of centrosome function, abnormal spindles and a failure to segregate chromosomes. These defects may result from the workings of a surveillance mechanism that acts to cull irreparable nuclei. ...
journal_title:Nature cell biology
pub_type: 评论,新闻
doi:10.1038/35000099
更新日期:2000-02-01 00:00:00
abstract::The asymmetric segregation of cell-fate determinants and the generation of daughter cells of different sizes rely on the correct orientation and position of the mitotic spindle. In the Drosophila embryo, the determinant Prospero is localized basally and is segregated equally to daughters of similar cell size during ep...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/71323
更新日期:2000-01-01 00:00:00
abstract::Here we investigate the role of the control of apoptosis in normal cell division. We show that interference with the expression or function of the apoptosis inhibitor survivin causes caspase-dependent cell death in the G2/M phase of the cell cycle, and a cell-division defect characterized by centrosome dysregulation, ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/70242
更新日期:1999-12-01 00:00:00
abstract::Programmed cell death (apoptosis) is used by multicellular organisms during development and to maintain homeostasis within mature tissues. One of the first genes shown to regulate apoptosis was bcl-2. Subsequently, a number of Bcl-2-related proteins have been identified. Despite overwhelming evidence that Bcl-2 protei...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/70237
更新日期:1999-12-01 00:00:00
abstract::Once considered to be contained, infectious diseases of bacterial origin are now making a comeback. A lack of innovative therapies and the appearance of drug-resistant pathogens are becoming increasingly serious problems. A better understanding of pathogen-host interactions at the cellular and molecular levels is nece...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/15620
更新日期:1999-11-01 00:00:00
abstract::The Semliki Forest virus capsid protein contains a chymotrypsin-like protease domain that must fold before it can autocatalytically cleave the protein from a larger polyprotein precursor. Here we analyse this cleavage in living mammalian and prokaryotic cells, and find that it occurs immediately after the emergence of...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/14032
更新日期:1999-10-01 00:00:00
abstract::Work over the past two years has led to a breakthrough in our understanding of the molecular basis of the directionality of the kinesin motor proteins. This breakthrough has come first from the reversal of directionality of the kinesin-related motor Ncd, followed closely by the reversal of kinesin's directionality and...
journal_title:Nature cell biology
pub_type: 杂志文章,评审
doi:10.1038/14113
更新日期:1999-10-01 00:00:00
abstract::The small GTPase ADP-ribosylation factor (ARF) regulates the structure and function of the Golgi complex through mechanisms that are understood only in part, and which include an ability to control the assembly of coat complexes and phospholipase D (PLD). Here we describe a new property of ARF, the ability to recruit ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/12993
更新日期:1999-09-01 00:00:00
abstract::Protein substrates of the proteasome must apparently be unfolded and translocated through a narrow channel to gain access to the proteolytic active sites of the enzyme. Protein folding in vivo is mediated by molecular chaperones. Here, to test for chaperone activity of the proteasome, we assay the reactivation of dena...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/12043
更新日期:1999-08-01 00:00:00
abstract::Adenomatous polyposis coli protein (APC) is an important tumour suppressor in the human colon epithelium. In a complex with glycogen synthase kinase-3 (GSK-3), APC binds to and destabilizes cytoplasmic ('free') beta-catenin. Here, using a yeast two-hybrid screen for proteins that bind to the Drosophila beta-catenin ho...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/11064
更新日期:1999-07-01 00:00:00
abstract::MAPKAP kinase 2 (MK2) is one of several kinases that are regulated through direct phosphorylation by p38 MAP kinase. By introducing a targeted mutation into the mouse MK2 gene, we have determined the physiological function of MK2 in vivo. Mice that lack MK2 show increased stress resistance and survive LPS-induced endo...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/10061
更新日期:1999-06-01 00:00:00
abstract::The GTPase dynamin plays an essential part in endocytosis by catalysing the fission of nascent clathrin-coated vesicles from the plasma membrane. Using preformed phosphatidylinositol-4,5-bisphosphate-containing lipid nanotubes as a membrane template for dynamin self-assembly, we investigate the conformational changes ...
journal_title:Nature cell biology
pub_type: 杂志文章
doi:10.1038/8997
更新日期:1999-05-01 00:00:00